"No doubt about it, Spike. There's definitely something strange going on at that school..." - Sci-Twi to Puppy Spike
Human Twilight Sparkle, also known as Sci-Twi (voiced by Tara Strong), is a former student of Crystal Prep Academy, currently student at Canterlot High School and one of the main protagonists of Twilight's Adventures Series. She is a former antagonist in Twilight and Kyle's Adventures of Equestria Girls: Friendship Games when she was transformed into her alter-ego, Midnight Sparkle. This version of Twilight looks considerably different from her Equestrian counterpart as she wears thick black glasses and her hair in a bun. She's used to be a student from Crystal Prep before being transferred to Canterlot High to study the magic of friendship.
In the Adventures Series, when Princess Twilight, Xion and her team came to Canterlot High once again for their late message because of Twilight's princess duties, they met her twin-sister at the same time, making all of The Rainbooms, Team Owl and Xion's team getting awkward moment.
Sci-Twi also started relationship with Kyle Akers after Twilight broke up with him.