(Princess) Twilight Sparkle (voiced by Tara Strong) is one of the main characters of My Little Pony: Friendship is Magic. She represents the element of magic. She's the student of Princess Celestia and makes friendship reports to her. Her best friends are Applejack, Fluttershy, Rarity, Rainbow Dash and Pinkie Pie. Her friend and assistant is Spike the dragon, and her big brother is Shining Armor, who is now married to her old foalsitter now older sister-in-law, Princess Cadence. She also helps her friends if something is troubling them, like Princess Celestia's sister Princess Luna.
- Twilight Sparkle and her friends will guest star in Jeffrey & Friends meet Bambi. At the end, she, along with Applejack, Fluttershy, Rainbow Dash, Rarity, Pinkie Pie, Spike and the Cutie Mark Crusaders, will join Jeffrey's team.
- In Jeffrey, Jaden & Friends' Storm Adventures of My Little Pony: Friendship is Magic - Magical Mystery Cure, Twilight Sparkle will get wings, become an alicorn and a princess, making her the second princess member on Jeffrey's side of the team.
- As the Masked Matter-Horn, she can generate energy beams with a variety of uses from her horn, such as energy blasts and freeze rays.
- Twilight teaches Xion history.