Tzekel-Kan (voiced by Armand Assante) is the main antagonist in The Road to El Dorado. He is the Mad High Priest who practically runs El Dorado, meaning that even Chief Tannabok had to answer to him. He is fanatically insane and has a strong fixation for human sacrifice, believing that such an act will please the gods. When Miguel and Tulio arrive in El Dorado and are believed to be gods, Tzekel-Kan and Chief Tannabok compete in order to please them. Before long, though, Tzekel-Kan discovers that they are in fact mortals and summons a giant stone jaguar to destroy them, but is ultimately defeated twice: the first time when the jaguar lands on top of him and they topple over into Xibalba, and the second time is when Miguel and Tulio thwart his plot to have the natives captured and/or killed.
- Tzekel-Kan will become Jeffrey's enemy in Jeffrey & Friends Go on The Road to El Dorado.
- Tzekel-Kan will become Aaron's enemy in Aaron Goes to The Road to El Dorado.
- Tzekel-Kan has faced Ed, Double D and Eddy previously.
- Tzekel-Kan will become Darkblade's enemy in Darkblade & The Road to El Dorado. He will join the Horde of Darkness and, in that way, get revenge on Darkblade and the Eds.
- tzekel-kan will become Kyle's enemy in Kyle Akers goes to The Road To El Dorado.