Yosemite Sam (originally voiced by Mel Blanc, currently voiced by Maurice LaMarche) is a loud-mouthed cowboy from the Looney Tunes and Merrie Melodies cartoons. He is a southern thief who always seems to run into Bugs Bunny who always outsmarts him. Although he is well-known as a cowboy, Sam has appeared as a pirate, a knight, a space alien, a Hessian soldier, a Confederate soldier, and even Robinson Crusoe. He is the Bugs and Daffy's Adventures team's second-biggest enemy, ever since the very first adventure, Bugs and Daffy's Adventures of The Lion King .
- Yosemite Sam makes an appearance in Jaden finds out Who Framed Roger Rabbit?